c-src-specific monoclonal antibody gd11 (Upstate Biotechnology Inc)
90
Structured Review
Upstate Biotechnology Inc
c-src-specific monoclonal antibody gd11
![Demonstration of <t>c-Src</t> inhibitory activity in the cardiocyte lysate. (A) c-Src autophosphorylation and enolase substrate phosphorylation measured in the absence (−) or presence (+) of the cardiocyte lysate (0.1 μg/μl). The positions of c-Src (60 kDa) and enolase (45 kDa) are indicated. (B) Dose response of the effects of cardiac lysate on c-Src activity determined with p34cdc2 synthetic peptide as the substrate. (C) Activity and amount of c-Src in the kinase reaction determined by autoradiography and Western blotting (W. Blot) with <t>c-Src</t> <t>monoclonal</t> antibody GD11, respectively. (D) Cardiocyte lysate was split into <10- and >10-kDa fractions as described in Materials and Methods. Top, c-Src autophosphorylation in the absence or presence of cardiocyte lysate, the <10-kDa fraction, and/or the >10-kDa fraction; bottom, similar experiment performed with c-Src preautophosphorylated with [γ-32P]ATP, where the effect of cardiocyte fractions was measured subsequently with unlabeled ATP. (E) The <10-kDa fraction was treated with buffer, subtilisin, or pronase and then used to study their effects on c-Src autophosphorylation in the absence or presence of the untreated >10-kDa (left) or <10-kDa (right) fraction.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4682/pmc00084682/pmc00084682__mb1090868001.jpg)
C Src Specific Monoclonal Antibody Gd11, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c-src-specific+monoclonal+antibody+gd11/full+length+human+purified+recombinant+pp60c+src/pmc00084682-147-0-7
Average 90 stars, based on 1 article reviews
C Src Specific Monoclonal Antibody Gd11, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c-src-specific+monoclonal+antibody+gd11/full+length+human+purified+recombinant+pp60c+src/pmc00084682-147-0-7
Average 90 stars, based on 1 article reviews
c-src-specific monoclonal antibody gd11 - by Bioz Stars,
2026-10
90/100 stars
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1) Product Images from "Inhibition of Src Family Kinases by a Combinatorial Action of 5?-AMP and Small Heat Shock Proteins, Identified from the Adult Heart"
Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5?-AMP and Small Heat Shock Proteins, Identified from the Adult Heart
Journal:
doi:
Figure Legend Snippet: Demonstration of c-Src inhibitory activity in the cardiocyte lysate. (A) c-Src autophosphorylation and enolase substrate phosphorylation measured in the absence (−) or presence (+) of the cardiocyte lysate (0.1 μg/μl). The positions of c-Src (60 kDa) and enolase (45 kDa) are indicated. (B) Dose response of the effects of cardiac lysate on c-Src activity determined with p34cdc2 synthetic peptide as the substrate. (C) Activity and amount of c-Src in the kinase reaction determined by autoradiography and Western blotting (W. Blot) with c-Src monoclonal antibody GD11, respectively. (D) Cardiocyte lysate was split into <10- and >10-kDa fractions as described in Materials and Methods. Top, c-Src autophosphorylation in the absence or presence of cardiocyte lysate, the <10-kDa fraction, and/or the >10-kDa fraction; bottom, similar experiment performed with c-Src preautophosphorylated with [γ-32P]ATP, where the effect of cardiocyte fractions was measured subsequently with unlabeled ATP. (E) The <10-kDa fraction was treated with buffer, subtilisin, or pronase and then used to study their effects on c-Src autophosphorylation in the absence or presence of the untreated >10-kDa (left) or <10-kDa (right) fraction.
Techniques Used: Activity Assay, Autoradiography, Western Blot
Related Articles
Activity Assay:Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5?-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5′-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: (Rockford, Ill.); [g-32P]ATP was obtained from Du Pont (Boston, Mass.); and the baculovirus expression system was from Novagen, Inc. (Madison, Wis.). .. Antibodies. Autoradiography:Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5?-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5′-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: (Rockford, Ill.); [g-32P]ATP was obtained from Du Pont (Boston, Mass.); and the baculovirus expression system was from Novagen, Inc. (Madison, Wis.). .. Antibodies. Western Blot:Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5?-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5′-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: (Rockford, Ill.); [g-32P]ATP was obtained from Du Pont (Boston, Mass.); and the baculovirus expression system was from Novagen, Inc. (Madison, Wis.). .. Antibodies. Purification:Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5?-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5′-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: (Rockford, Ill.); [g-32P]ATP was obtained from Du Pont (Boston, Mass.); and the baculovirus expression system was from Novagen, Inc. (Madison, Wis.). .. Antibodies. Recombinant:Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5?-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5′-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: (Rockford, Ill.); [g-32P]ATP was obtained from Du Pont (Boston, Mass.); and the baculovirus expression system was from Novagen, Inc. (Madison, Wis.). .. Antibodies. Concentration Assay:Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5?-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5′-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: (Rockford, Ill.); [g-32P]ATP was obtained from Du Pont (Boston, Mass.); and the baculovirus expression system was from Novagen, Inc. (Madison, Wis.). .. Antibodies. Inhibition:Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5?-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5′-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: (Rockford, Ill.); [g-32P]ATP was obtained from Du Pont (Boston, Mass.); and the baculovirus expression system was from Novagen, Inc. (Madison, Wis.). .. Antibodies. Chromatography:Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5?-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5′-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: (Rockford, Ill.); [g-32P]ATP was obtained from Du Pont (Boston, Mass.); and the baculovirus expression system was from Novagen, Inc. (Madison, Wis.). .. Antibodies. Sequencing:Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5?-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5′-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: (Rockford, Ill.); [g-32P]ATP was obtained from Du Pont (Boston, Mass.); and the baculovirus expression system was from Novagen, Inc. (Madison, Wis.). .. Antibodies. Labeling:Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5?-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5′-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: (Rockford, Ill.); [g-32P]ATP was obtained from Du Pont (Boston, Mass.); and the baculovirus expression system was from Novagen, Inc. (Madison, Wis.). .. Antibodies. Mass Spectrometry:Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5?-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5′-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: (Rockford, Ill.); [g-32P]ATP was obtained from Du Pont (Boston, Mass.); and the baculovirus expression system was from Novagen, Inc. (Madison, Wis.). .. Antibodies. Kinase Assay:Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5?-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5′-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: (Rockford, Ill.); [g-32P]ATP was obtained from Du Pont (Boston, Mass.); and the baculovirus expression system was from Novagen, Inc. (Madison, Wis.). .. Antibodies. Transfection:Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5?-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5′-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: (Rockford, Ill.); [g-32P]ATP was obtained from Du Pont (Boston, Mass.); and the baculovirus expression system was from Novagen, Inc. (Madison, Wis.). .. Antibodies. Plasmid Preparation:Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5?-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5′-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: (Rockford, Ill.); [g-32P]ATP was obtained from Du Pont (Boston, Mass.); and the baculovirus expression system was from Novagen, Inc. (Madison, Wis.). .. Antibodies. Immunoprecipitation:Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5?-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5′-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: (Rockford, Ill.); [g-32P]ATP was obtained from Du Pont (Boston, Mass.); and the baculovirus expression system was from Novagen, Inc. (Madison, Wis.). .. Antibodies. Phospho-proteomics:Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5?-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: Article Title: Inhibition of Src Family Kinases by a Combinatorial Action of 5′-AMP and Small Heat Shock Proteins, Identified from the Adult Heart Article Snippet: (Rockford, Ill.); [g-32P]ATP was obtained from Du Pont (Boston, Mass.); and the baculovirus expression system was from Novagen, Inc. (Madison, Wis.). .. Antibodies. |